Monitoring the immune response to vaccination with an inactivated vaccine associated to bovine neonatal pancytopenia by deep sequencing transcriptome analysis in cattle

Demasius, Wiebke; Weikard, Rosemarie; Hadlich, Frieder; Müller, Kerstin Elisabeth; Kühn, Christa

doi:10.1186/1297-9716-44-93

Veterinary Research

Table 1 Overview of the alignments of reads per sample.

From: Monitoring the immune response to vaccination with an inactivated vaccine associated to bovine neonatal pancytopenia by deep sequencing transcriptome analysis in cattle

Individual	Sampling	Breed	Total number of fragments	Uniquely counted, mapped reads with < 3 mismatches	% of reads uniquely mapping, < 3 mismatches	% of reads mapping to globin clusters
cow 1	before vacc	Cha × GH F₂	44 776 690	77 918 387	87.01	1.04
cow 1	after vacc	Cha × GH F₂	44 627 365	78 360 907	87.79	2.01
cow 2	before vacc	Cha × GH F₂	43 990 812	77 993 272	88.65	0.07
cow 2	after vacc	Cha × GH F₂	44 636 963	77 742 662	87.08	0.23
cow 3	before vacc	Cha × GH F₂	40 798 596	71 377 966	87.48	0.10
cow 3	after vacc	Cha × GH F₂	37 816 325	65 542 541	86.66	0.11
cow 4	before vacc	Cha × GH F₂	35 447 535	61 851 369	87.24	0.18
cow 4	after vacc	Cha × GH F₂	39 030 040	69 141 268	88.57	0.16
cow 5	before vacc	GH	34 889 160	61 093 146	87.55	0.40
cow 5	after vacc	GH	33 543 345	59 711 466	89.01	1.33
cow 6	before vacc	Cha × GH F₂	42 232 327	73 237 641	86.71	0.10
cow 6	after vacc	Cha × GH F₂	41 569 088	71 882 069	86.46	0.26
cow 7	before vacc	Cha × GH F₂	40 572 312	70 148 894	86.45	3.06
cow 7	after vacc	Cha × GH F₂	44 155 006	77 074 387	87.28	0.92
cow 8	before vacc	Cha × GH F₂	35 704 593	61 371 734	85.94	0.51
cow 8	after vacc	Cha × GH F₂	37 375 624	65 532 890	87.67	0.86
cow 9	before vacc	Cha × GH F₂	33 125 544	57 825 014	87.28	0.37
cow 9	after vacc	Cha × GH F₂	38 731 113	67 755 124	87.47	0.05
cow 10	before vacc	Cha × GH F₂	36 740 555	62 541 431	85.11	1.10
cow 10	after vacc	Cha × GH F₂	38 024 182	67 142 881	88.29	7.14
cow 11	before vacc	Cha × GH F₂	36 549 423	63 893 567	87.41	2.17
cow 11	after vacc	Cha × GH F₂	39 502 827	69 593 787	88.09	0.56
cow 12	before vacc	Cha × GH F₂	45 701 139	79 680 037	87.18	0.16
cow 12	after vacc	Cha × GH F₂	43 718 688	77 239 417	88.34	1.18
Total			953 259 252	1 665 651 857

Cha, Charolais; GH, German Holstein; vacc, vaccination.

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ISSN: 1297-9716

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