H3N2 canine influenza virus causes severe morbidity in dogs with induction of genes related to inflammation and apoptosis

Kang, Young Myong; Kim, Heui Man; Ku, Keun Bon; Park, Eun Hye; Yum, Jung; Seo, Sang Heui

doi:10.1186/1297-9716-44-92

Veterinary Research

Table 1 Real-time PCR primer sequences

From: H3N2 canine influenza virus causes severe morbidity in dogs with induction of genes related to inflammation and apoptosis

Group	Gene GAPDH	Forward primer TCA ACG GAT TTG GCC GTA TTG G	Reverse primer TGA AGG GGT CAT TGA TGG CG
TLR	TLR2	TGT TGT TGG GCA ACT GAA AA	TTA AAC AAG TGG GGC AAA GG
NK cell	IL15	AGT AAC CGC GAT GAA GTG CT	CTT TGC ATC CCG TTT CAG TT
NK cell	FCIGR	CCT GGT GAT GGC ACT CCT AT	GGG TTG TGG ATC CAG AGA GA
Macrophage	MARCO	CCA GGA CTT TTG GCC ATT TA	TTC TCT TCT TGG GCT TTG GA
Macrophage	MMP12	GTC ACT GCT TCG GGT ACC AT	GAT GCA TCC AGT TTC CCA GT
Neutrophil	CSF2	AAC ATC CCC TTT GAC TGC TG	AAG TCC ATG CCC CCT CTA CT
Neutrophil	SLPI	CCT TGG ACT GTG GAA GGT GT	ACC TGC CAG GCT TCA TTC TA
NO and ROS	GCLM	GGC GCA GGT AAA ACC AAA TA	GCT TTC CTG AAG GGC TTC TT
	HSP90AA1	GTC AGT GAC GAT GAG GCT GA	GTG ATG TCG TCA GGG TTC CT
	ADM	CGA CGT CTC AGA CCT TCT CC	CCA CGA CTT ACA GCC CAT TT
	SOD2	TGT TGT TGG GCA ACT GAA AA	TTA AAC AAG TGG GGC AAA GG
IFN	IFIT3	TTC CCC ACA AGA GCA AAA TC	GCA GGA AAA AGC TGC CTA TG
	ISG20	CGA TGG AGC TTT ACC GAC TC	ATG CAT TTC CCA AAA GCA AG
	MX1	GGA GGC TCT GTC AGG AGT TG	TGA CTG ATC CCC TGT CCT TC
Cytokine and chemokine	IL15	AGT AAC CGC GAT GAA GTG CT	CTT TGC ATC CCG TTT CAG TT
	C3	CGC TAC CAG AAC CTG AGG AG	CGG ACG ACA GGA GGT ACA TT
	IL10RB	GCC TTC ACG GAA TGT GAT TT	TAG GGG CTA AGA AAC GCA GA
	IL1R2	ATG GGT GTT TCA GCC TTC AC	CTG GAC CCA CAT TCT CGT CT
	CXCL10	TGA ACC AAA GTG CTG TTC TTA TTT	ACG ATG GAC TTG CAG GAA TC
DC	TAP1	CCA TGG CTC TCA CTA GCA CA	GCA GGC TGG AGC TCA ATT AC
DC	MX1	GGA GGC TCT GTC AGG AGT TG	TGA CTG ATC CCC TGT CCT TC
Apoptosis	TNFSF13	GGG CGA AAC TTA GCC TCT CT	GGT TGA GGT CAA ACC CAG AA
	NME5	AAG GGC AAT TTA TGG CAC AG	CCG CTG GTT TCT CCT TAC AA
	PYCARD	CTG CAG GAG ACC TCA CAC AA	TCC TCA TTT TCC CTG GAT TG
TH1	SPP1	TTG CAG TGA TTT GCT TTT GC	CAG ACT CGT TGG AGT CGT CA
TH2	CCL5	GCT GCT TTG CCT ACA TTT CC	TCA GGT TCC AGA TGC CCT AC
	CCL7	CCC ATC CAG AAG CTG AAG AG	GCT TGG GTT TTC TTG TCC AG

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ISSN: 1297-9716

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