Figure 2

Clinical signs and viral titer in the infected dogs, and aerosol transmission of canine H3N2 influenza virus. Dogs (Korean mongrel, n = 3) were i.n. infected with A/Korea/S1/Canine/2012 (H3N2), and the rectal body temperature (A) and body weight (B) were measured for 14 days after infection. Clinical scores were measured as described in the Materials and methods (C), and the viral titer (D) of swabs performed daily from tracheas and rectum was determined by log₁₀ EID₅₀/mL. To study aerosol transmission (E) of the canine H3N2 influenza virus, 2 dogs (Korean mongrel) infected with A/Korea/S1/Canine/2012 (H3N2) in a cage were placed 90.0 cm apart from a cage containing 3 naïve dogs 1 day after infection. The viral titer in the nasal and rectal swabs performed on 3 and 5 days after transmission setup was determined by log₁₀ EID₅₀/mL.