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Figure 7 | Veterinary Research

Figure 7

From: Potential of recombinant inorganic pyrophosphatase antigen as a new vaccine candidate against Baylisascaris schroederi in mice

Figure 7

Serum IgG and IgG subclass (IgG1 and IgG2a) responses induced by r Bsc -PYP-1 in mice. Mice were tested for serum IgG and its subclasses before and after challenge with B. schroederi infective embryonated eggs either at 5 wpv (trial II) or at 6 wpv (trial I). Mice were subcutaneously vaccinated three times with rBsc-PYP-1 mixed with FCA in PBS at 14-day intervals, and serum samples were collected from the tail vein after each vaccination and at 0 (challenge or 6 wpv), 1, 3, 5, 6 and 7 wpc. Endpoint titers of anti-rBsc-PYP-1 IgG (A) and IgG-subclass (B) antibodies from immunized and control mice were determined by ELISA. Values are expressed as the mean ± SD for each group of six mice. Vaccination times (V1, V2, and V3) and challenge (C) are marked with black arrows, and asterisks in A and B indicate statistically significant differences between the rBsc-PYP-1/FCA/PBS vaccination group and two control groups (PBS plus FCA or PBS alone) (P < 0.001). Error bars represent SD.

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