SDS-PAGE and Western blotting analysis of purified r Bcs -PYP-1. M, molecular mass marker in kDa; lane 1, purified rBcs-PYP-1 after dialysis; lanes 2–5: purified rBsc-PYP-1 was probed with rabbit immune serum against B. schroederi (experimental group, lane 2), naive rabbit serum (negative control, lane 3), anti-rBsc-PYP-1 mouse serum (positive control, lane 4) and pre-immune mouse serum (negative control, lane 5), respectively. Two micrograms of protein was loaded in each lane of an SDS-10% polyacrylamide gel, subjected to electrophoresis and blotted onto nitrocellulose membranes. The protein was stained with Coomassie Blue R250 in the gel (lane 1), while the protein bound to serum samples was detected using NBT/BCIP in the Western blot (lanes 2–5). The arrow indicates the location of rBsc-PYP-1-specific bands.