Figure 1

Sequence alignment and phylogenetic analysis of Bsc -PYP-1 with homologous PPases. (A) Alignment of the deduced amino acid sequence of Bsc-PYP-1 with those of homologous PPases from other species. The following sequences were retrieved from the GenBank protein sequence database (accession numbers are indicated in parentheses) and aligned using the ClustalW2 program: B. schroederi (GQ859591), A. suum (BAC66617), B. malayi (EDP36300), L. loa (EFO25093), C. elegans (NP_001023073), C. briggsae (XP_002633752), C. remanei (EFP04160) and T. spiralis (EFV52164). Regions of identity (*), strong similarity (:) and weak similarity (.) are indicated. Gaps, marked by hyphens, are introduced for better alignment. The putative PPase signature motif is enclosed in the box, and 13 well-conserved residues in all family I soluble PPases are marked with black stars. The predicted signal peptide is underlined. Percentages of sequence similarity with respect to Bsc-PYP-1 are shown at the end of each sequence. (B) Phylogenetic analysis of the full-length amino acid sequences of Bsc-PYP-1 and homologous PPases from the eight nematodes mentioned in (A). The tree was constructed by the NJ method and plotted with MEGA 3.1. Bootstrap values are indicated at the nodes (1000 replications). The scale indicates an estimate of substitutions per site, using the optimized model setting.