Figure 6

Detection of IDO in the lung (a,b) and small intestine (c,d) of T . gondii infected mice. BALB/c and C57BL/6 mice were orally infected with 5 T. gondii cysts and treated with ZnPPIX (10 mg/kg/day), hemin (5 mg/kg/day) or vehicle (Na₂CO₃ + NaCl) and analyzed at day 12 pi. Protein extracts obtained from tissue samples were submitted to Western blotting assay for detection of IDO expression. Representative Western blotting for IDO and β-actin in the lung (a) and small intestine (c) and densitometric analyses showing the relative density of the respective proteins obtained by the ratio between IDO/β-actin bands in the lung (b) and small intestine (d). Data are representative of at least two independent experiments of five mice per group. ^*Statistically significant differences between experimental conditions in the same lineage of mice (ANOVA and Bonferroni multiple comparison post-test, P < 0.05). ^#Statistically significant differences between the two mouse lineages submitted to the same treatment conditions (Student’s t test, P < 0.05).