Cytokines, HO-1 and IDO mRNA expression in the lung of T . gondii infected mice. BALB/c and C57BL/6 mice were infected with 5 T. gondii cysts by oral route and treated with ZnPPIX (10 mg/kg/day), hemin (5 mg/kg/day) or vehicle (Na2CO3 + NaCl) and analyzed at 12 dpi. The relative levels of TNF-α (a), IFN-γ (b), TGF-β (c), IL-10 (d), HO-1 (e) and IDO (f) gene expression were calculated by reference to the β-actin in each sample, using the threshold cycle (Ct) method. Data are representative of at least two independent experiments of five mice per group. *Statistically significant differences between experimental conditions in the same lineage of mice (ANOVA and Bonferroni multiple comparison post-test, P < 0.05). #Statistically significant differences between the two mouse lineages submitted to the same treatment conditions (Student’s t test, P < 0.05).