Quantification of RVA RNA copy numbers by SYBR Green real-time RT-PCR in the feces, serum, mesenteric lymph node (MLN), liver, lung, and choroid plexus. (A) The sequential changes of viral RNA copy numbers of a porcine G5P strain in the feces, serum, MLN, liver, lung and choroid plexus sampled from virus-infected piglets. Fecal specimens sampled from porcine G5P K71 strain-inoculated calves were also examined by real-time RT-PCR. Note that viral RNA copy numbers in the feces from virus-inoculated calves were markedly lower than those from virus-inoculated piglets. (B and C) The sequential changes of viral RNA copy numbers in the feces, serum, MLN, liver, lung, and choroid plexus sampled from virus-inoculated piglets (B) or calves (C) were similar to each other. The geometric means of virus RNA copy numbers per mg of each sample are displayed.