Distribution of RVA antigen-positive cells of porcine and bovine G5P strains in the duodenum. (A and B) Duodenum sampled at 3 dpi from a piglet (A) and a calf (B) infected with bovine K5 strain had antigen-positive cells (arrows) in the villi. (C and D) K71 RVA antigen-positive cells (arrows) were detected in the villi of duodenum sampled from a virus inoculated piglet at 1 dpi (C), but not in those from a virus inoculated calf (D). Indirect immunofluorescence assay with monoclonal antibody against the VP6 protein of strain OSU. Bars denote 100 μm.