Figure 3From: Increase in chemokines CXCL10 and CCL2 in blood from pigs infected with high compared to low virulence African swine fever virus isolatesWestern blot to detect CXCL10 in plasma samples from ASFV infected and uninfected pigs. Levels of CXCL10 protein were measured in de-fibrinated plasma from pigs uninfected or infected with ASFV isolates Benin 97/1. Samples were separated by SDS-PAGE and Western blotting followed by immunodetection using a polyclonal antibody against porcine CXCL10 followed by a secondary antibody couple to HRP. Bound antibodies were detected by enhanced chemiluminesence. The results from samples collected from individual pigs (numbered below) at different dpi are shown. The position of a 12 kDa molecular weight marker run in parallel is indicated.Back to article page