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Table 2 Detection of BTV RNA by RT-qPCR in blood and tissues of lambs.

From: Transplacental transmission of field and rescued strains of BTV-2 and BTV-8 in experimentally infected sheep

Lamb  Virus Blood sample Blood sample Blood sample Spleen Mesenteric lymph nodes Brain Cotyledons
   11 22 33     
1 None - - - - - - -
2   - - - - - - -
3   - - - - - - -
4   19.32 21.3 18.5 24.86 29.71 27.28 N.D.
5 BTV-2 wt - - - - - - 28.93
6   18.08 N.D. N.D. 21.87 26.83 24.98 23.54
7   - - - - - - N.D.
8   20.82 18.88 18.91 23.48 30.84 26.87 24.25
9   - - - - - - 29.94
10   - - - N.D. N.D. N.D. 25.96
11 BTV-8 wt - - - - - - 35.05
12   - - - - - - N.D.
13   - - - - - - 28.49
14   - - - - - - 27.65
15   N.D.4 N.D.4 N.D.4 19.99 27.41 25.96 22.72
16   - - N.D. - - - N.D.
17 BTV-8 rg - - - - - - -
18   - - - - - - 33.13
19   - - - - - - 25.72
20   - - - - - - 34.26
21   - - - - - - N.D.
22   - - - - - - 26.61
23 BTV-2 rg 19.53 18.12 19.67 26.23 31.8 29.47 22.16
24   - - - - - - 31.02
25   - N.D. N.D. N.D. - - N.D.
26   22.47 21.12 20.24 26.57 30.62 30.17 25.91
27   21.1 19.28 19.29 26.08 30.75 29.47 24.49
28   - - - - - - N.D.
  1. 1: Blood sample 1 was taken soon after birth before colostrum was received.
  2. 2: Blood sample 2 was normally taken at 3dpi but was delayed in some samples.
  3. 3: Blood sample 3 was normally taken at 7 dpi but was delayed in some samples.
  4. 4: Lamb 15 died at birth.
  5. - indicates no Ct value obtained by 50 cycles.
  6. N.D. = not determined.