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Table 2 Detection of BTV RNA by RT-qPCR in blood and tissues of lambs.

From: Transplacental transmission of field and rescued strains of BTV-2 and BTV-8 in experimentally infected sheep

Lamb

 Virus

Blood sample

Blood sample

Blood sample

Spleen

Mesenteric lymph nodes

Brain

Cotyledons

  

11

22

33

    

1

None

-

-

-

-

-

-

-

2

 

-

-

-

-

-

-

-

3

 

-

-

-

-

-

-

-

4

 

19.32

21.3

18.5

24.86

29.71

27.28

N.D.

5

BTV-2 wt

-

-

-

-

-

-

28.93

6

 

18.08

N.D.

N.D.

21.87

26.83

24.98

23.54

7

 

-

-

-

-

-

-

N.D.

8

 

20.82

18.88

18.91

23.48

30.84

26.87

24.25

9

 

-

-

-

-

-

-

29.94

10

 

-

-

-

N.D.

N.D.

N.D.

25.96

11

BTV-8 wt

-

-

-

-

-

-

35.05

12

 

-

-

-

-

-

-

N.D.

13

 

-

-

-

-

-

-

28.49

14

 

-

-

-

-

-

-

27.65

15

 

N.D.4

N.D.4

N.D.4

19.99

27.41

25.96

22.72

16

 

-

-

N.D.

-

-

-

N.D.

17

BTV-8 rg

-

-

-

-

-

-

-

18

 

-

-

-

-

-

-

33.13

19

 

-

-

-

-

-

-

25.72

20

 

-

-

-

-

-

-

34.26

21

 

-

-

-

-

-

-

N.D.

22

 

-

-

-

-

-

-

26.61

23

BTV-2 rg

19.53

18.12

19.67

26.23

31.8

29.47

22.16

24

 

-

-

-

-

-

-

31.02

25

 

-

N.D.

N.D.

N.D.

-

-

N.D.

26

 

22.47

21.12

20.24

26.57

30.62

30.17

25.91

27

 

21.1

19.28

19.29

26.08

30.75

29.47

24.49

28

 

-

-

-

-

-

-

N.D.

  1. 1: Blood sample 1 was taken soon after birth before colostrum was received.
  2. 2: Blood sample 2 was normally taken at 3dpi but was delayed in some samples.
  3. 3: Blood sample 3 was normally taken at 7 dpi but was delayed in some samples.
  4. 4: Lamb 15 died at birth.
  5. - indicates no Ct value obtained by 50 cycles.
  6. N.D. = not determined.
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Veterinary Research

ISSN: 1297-9716