Figure 1From: Establishment of feline intestinal epithelial cell cultures for the propagation and study of feline enteric coronavirusesMorphological features and immunocytochemical characterization of the primary ileum (A-C) and colon (D-F) cultures. (A) Epithelial cells were isolated in cell clusters. (B, D) Polygonal cells started to spread from these clusters giving rise to several foci of cells. (E) (Sub) confluent layers were reached 3–4 days after seeding due to a restricted proliferation of the cells. (C, F) Double-immunostainings against cytokeratin (red) and vimentin (green) filaments 4 days after isolation, confirming the epithelial nature of the polygonal cells.Back to article page