Morphological features and immunocytochemical characterization of the primary ileum (A-C) and colon (D-F) cultures. (A) Epithelial cells were isolated in cell clusters. (B, D) Polygonal cells started to spread from these clusters giving rise to several foci of cells. (E) (Sub) confluent layers were reached 3–4 days after seeding due to a restricted proliferation of the cells. (C, F) Double-immunostainings against cytokeratin (red) and vimentin (green) filaments 4 days after isolation, confirming the epithelial nature of the polygonal cells.