Skip to main content
Figure 5 | Veterinary Research

Figure 5

From: Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products

Figure 5

Gene expression in helminth-free PBMC cultures following stimulation with Con A ± Tci - L4 - ES. PBMC from helminth-naïve lambs cultured for 72 h with PBS alone (PBS), 5 μg/mL Con A alone (ConA + PBS), 5 μg/mL Con A + 30 μg/mL Tci-L4-ES (ConA + ES) or 5 μg/mL Con A + 30 μg/mL heat-inactivated Tci-L4-ES (ConA + HiES). Relative gene expression of IL-10 (A), TGF1 (B), IL-4 (C) and IFN(D) was determined by quantitative RT-PCR. Data represents mean ± SEM from three replicate cultures from three helminth-free lambs. **P < 0.01, ***P < 0.001 (one way ANOVA followed by the Tukey post hoc test for pairwise comparison of means).

Back to article page