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Figure 2 | Veterinary Research

Figure 2

From: Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products

Figure 2

Expression of Foxp3 and CD25 by CD4+T cells stimulated with Con A ± Tci - L4 - ES. (A) Representative plots of Foxp3 and CD25 expression by CD4+ T cells from PBMC from helminth-naïve lambs cultured for 72 h with PBS alone (PBS), 5 μg/mL Con A alone (ConA + PBS), 5 μg/mL Con A + 30 μg/mL Tci-L4-ES (ConA + ES) or 5 μg/mL Con A + 30 μg/mL heat-inactivated Tci-L4-ES (ConA + HiES). Numbers indicate the percentage of CD4+ cells co-expressing Foxp3 (top panel) or CD25 (middle panel) and the percentage of CD4+CD25+ cells co-expressing Foxp3 (lower panel). (B) Percentages of total PBMC, CD4+ cells and CD4+CD25+ cells co-expressing Foxp3, and percentages of total PBMC and CD4+ cells co-expressing CD25. Data represents mean ± SEM from three replicate cultures from three helminth-free lambs. *P < 0.05, ***P < 0.001 (one way ANOVA followed by the Tukey post hoc test for pairwise comparison of means).

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