Table 4 Number of fish detected with virus by IHC and qPCR at 56 days post challenge
Vaccine group | Tissue | IHC | qPCR | Log of viral copy numbers in tissues detected by IHC ( Pathology*) | ||
|---|---|---|---|---|---|---|
No. of infected fish | % | No. of infected fish | % | |||
Control | Headkidney | 3/6 | 50.0% | 10/10 | 100.0% | 6.78, 6.52, 6.55 |
Pancreas | 3/6 | 50.0% | 10/10 | 100.0% | 6.78, 6.54, 7.12 | |
Liver | 3/6 | 50.0% | 10/10 | 100.0% | 6.57, 6.52, 6.72 | |
Spleen | 3/6 | 50.0% | 10/10 | 100.0% | 6.93, 6.85, 6.51 | |
Gills | 2/6 | 33.3% | ND | - | - | |
Muscle | 2/6 | 33.3% | ND | - | - | |
Heart | 0/6 | 00.0% | ND | - | - | |
Pyloric ceca | 0/6 | 00.0% | ND | - | - | |
LoAg | Headkidney | 3/6 | 50.0% | 10/10 | 100.0% | 6.78, 6.62, 6.55 |
Pancreas | 3/6 | 50.0% | 10/10 | 100.0% | 7.18, 6.54, 7.12† | |
Liver | 3/6 | 50.0% | 8/10 | 80.0% | 6.57, 7.22†, 6.72 | |
Spleen | 3/6 | 50.0% | 10/10 | 100.0% | 6.93, 6.85, 6.51 | |
Gills | 0/6 | 00.0% | ND | - | - | |
Muscle | 2/6 | 33.3% | ND | - | - | |
Heart | 0/6 | 00.0% | ND | - | - | |
Pyloric ceca | 0/6 | 00.0% | ND | - | - | |
HiAg | Headkidney | 0/6 | 00.0% | 10/10 | 100.0% | - |
Pancreas | 0/6 | 00.0% | 9/10 | 90.0% | - | |
Liver | 0/6 | 00.0% | 6/10 | 60.0% | - | |
Spleen | 0/6 | 00.0% | 10/10 | 100.0% | - | |
Gills | 0/6 | 00.0% | ND | - | - | |
Muscle | 0/6 | 00.0% | ND | - | - | |
Heart | 0/6 | 00.0% | ND | - | - | |
Pyloric ceca | 0/6 | 00.0% | ND | - | - | |