Table 1 Primers used for quantitative PCR
Gene | GenBank | Sequence 5´-3´ | Tm (°C) | Size (bp) | |
|---|---|---|---|---|---|
β-actin | BT047241.2 | F | CCAGTCCTGCTCACTGAGGC | 63.5 | 75bp |
R | GGTCTCAAACATGATCTGGGTCA | 63.5 | |||
ELFα | AF321836 | F | GCTGTGCGTGACATGAGG | 58.2 | 88bp |
R | ACTTTGTGACCTTGCCGC | 58.2 | |||
Mx (1) | SSU66475 | F | TGCAACCACAGAGGCTTTGAA | 57.9 | 78bp |
R | GGCTTGGTCAGGATGCCTAAT | 57.9 | |||
IFNα (2) | NM_001123570 | F | TGGGAGGAGATATCACAAAGC | 57.9 | 163bp |
R | TCCCAGGTGACAGATTTCAT | 57.9 | |||
ProbeIPNVsegB | AY379740 | 6FAM-CCGGATTCCTAGACGAC | |||
F | GACTGGAGGTAAAAGGCATCGA | 60.3 | 68bp | ||
R | CCGAACTCCGACATGGTGTT | 59.4 |