Expression of IFN-γ in splenic and MLN CD8+and CD4+T cells. Scatter plots of frequency of CD8+IFN-γ+ T cells (a and b) or CD4+IFN-γ+ T cells (c and d), as indicated, in the MNL (a and c) and spleen (b and d) of control (PBS) or N. caninum-infected mice (NcT), 4 and 7 days upon i.g. challenge. Each symbol represents an individual mouse; horizontal bars correspond to mean values of the respective group. Five mice per group were used. This is one representative result of three independent experiments. Statistical significance between groups is indicated above symbols (*P<0.05; **P<0.01).