Figure 1
From: Experimental transmission of enzootic nasal adenocarcinoma in sheep
Reverse transcriptase activity assay and immunoblot analysis of ENA inoculum. The reverse transcriptase activity of the ENA inoculum was quantified using a fluorogenic based assay (A) and compared to a mock inoculum comprised of concentrated filtered supernatant from a sheep skin fibroblast cell line. (B) Immunoblot analysis of cell lysate from normal sheep nasal tissue (lane 1), ENA inoculum (lane 2) and cell lysate from a naturally occurring ENA tumor (lane 3) with envelope surface subunit (Env SU) and capsid (CA) specific antibodies. M indicates molecular weight marker.