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Table 2 Statistical analysis of mRNA expression levels.

From: Diversity in bacterium-host interactions within the species Helicobacter heilmannii sensu stricto

   Antrum   Fundus
  IL-1β   IFN-γ   H+/K+ATPase   Gastrin  
  Mean Ct-Ctrefa p-valueb Mean Ct-Ctref p-value Mean Ct-Ctref p-value Mean Ct-Ctref p-value
ASB1 6.92 ± 0.29 * 0.031 6.87 ± 0.60 1.000 3.70 ± 2.11 * 0.050 7.20 ± 1.68 0.101
ASB2 7.48 ± 1.42 0.231 8.15 ± 1.26 1.000 2.42 ± 5.01 0.513 5.94 ± 2.49 * 0.050
ASB3 6.55 ± 0.80 * 0.008 7.54 ± 0.37 1.000 3.49 ± 2.28 * 0.050 7.71 ± 1.17 0.101
ASB6 6.12 ± 0.67 * 0.001 7.99 ± 0.90 1.000 2.23 ± 1.99 * 0.050 5.33 ± 2.39 * 0.025
ASB7 10.25 ± 0.37 1.000 7.98 ± 0.52 1.000 0.45 ± 2.51 0.724 7.12 ± 1.54 0.180
ASB9 9.03 ± 2.54 1.000 8.55 ± 0.71 1.000 0.79 ± 0.03 0.564 8.11 ± 1.58 1.000
ASB11 7.47 ± 0.15 0.499 10.45 ± 0.95 * 0.004 1.32 ± 1.45 0.248 7.67 ± 1.25 0.289
ASB13 7.72 ± 1.17 0.523 9.54 ± 1.55 * 0.044 3.80 ± 0.34 0.083 8.10 ± 0.93 0.456
ASB14 7.07 ± 0.52 0.054 8.51 ± 0.97 1.000 3.81 ± 3.11 0.127 7.77 ± 2.96 0.881
Negative Control 9.71 ± 1.13 - 7.08 ± 0.65 - 0.15 ± 1.82 - 8.70 ± 0.62 -
  1. Shown is the statistical analysis of IL-1β, IFN-γ and H+/K+ ATPase mRNA expression in the antrum and gastrin mRNA expression in the fundus of the gerbil stomach at 9 weeks after experimental infection. Cytokine expression was analyzed by analysis of variance with a Bonferroni post hoc test. H+/K+ ATPase and gastrin gene expression were compared between different infected groups and controls using Kruskall-Wallis analysis, followed by a Mann-Whitney U test. Differences were considered statistically significant at p ≤ 0.05. SPSS Statistics 21 software (IBM) was used for all analyses.
  2. a Mean Ct-Ctref: For each experimental group, the mean of the normalized Ct-values ± standard deviation are shown.
  3. bp-value: the exact p-values are given.
  4. * Statistically significant differences compared to the uninfected control group (p ≤ 0.05).