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Table 1 Primer pairs.

From: Diversity in bacterium-host interactions within the species Helicobacter heilmannii sensu stricto

  Primers Sequence (5’ → 3’)
Cytokines IL-1β FW23 GGC AGG TGG TAT CGC TCA TC
IL-1β RV23 CAC CTT GGA TTT GAC TTC TA
IL-5 FW AGA GAA GTG TGG CGA GGA GAG ACG
IL-5 RV ACA GGG CAA TCC CTT CAT CGG
IL-6 FW CAA AGC CAG AGC CAT TCA GAG
IL-6 RV GCC ATT CCG TCT GTG ACT CCA GTT TCT CC
IL-10 FW GGT TGC CAA GCC TTA TCA GA
IL-10 RV GCT GCA TTC TGA GGG TCT TC
IL-12p40 FW GAC ACG ACC TCC ACC AAA GT
IL-12p40 RV CAT TCT GGG ACT GGA CCC TA
IL-17 FW23 AGC TCC AGA GGC CCT CGG AC
IL-17 RV23 AGG ACC AGG ATC TCT TGC TG
IFN-γ FW24 CCA TGA ACG CTA CAC ACT GCA TC
IFN-γ RV24 GAA GTA GAA AGA GAC AAT CTG G
TNF-α FW23 GCT CCC CCA GAA GTC GGC G
TNF-α RV23 CTT GGT GGT TGG GTA CGA CA
Gastrin Gastrin FW25 GCC CTG GAA CCG CAA CA
Gastrin RV25 TTC TTG GAC AGG TCT GCT TTG AA
H + /K + ATPase (parietal cells) ATP4b FW GGG GGT AAC CTT GAG ACC TGA TG
ATP4b RV AAG AAG TAC CTT TCC GAC GTG CAG
Reference genes GAPDH FW26 AAC GGC ACA GTC AAG GCT GAG AAC G
GAPDH RV26 CAA CAT ACT CGG CAC CGG CAT CG
HPRT FW27 CTC ATG GAC TGA TTA TGG ACA G
HPRT RV27 AGC TGA GAG ATC ATC TCC ACC AAT
β-actin FW28 CCA AGG CCA ACC GCG AGA TGA C
β-actin RV28 AGG GTA CAT GGT GGT GCC GCC AGA C
  1. Primer pairs used for measuring the mRNA expression levels of IL-1β, IL-5, IL-6, IL-10, IL-12p40, IL-17, IFN-γ, TNF-α, gastrin and H+/K+ ATPase are shown. The housekeeping genes GAPDH, β-actin and HPRT were included as reference genes.