Diversity in bacterium-host interactions within the species Helicobacter heilmannii sensu stricto

Table 1 Primer pairs.

	Primers	Sequence (5’ → 3’)
Cytokines	IL-1β FW²³	GGC AGG TGG TAT CGC TCA TC
	IL-1β RV²³	CAC CTT GGA TTT GAC TTC TA
	IL-5 FW	AGA GAA GTG TGG CGA GGA GAG ACG
	IL-5 RV	ACA GGG CAA TCC CTT CAT CGG
	IL-6 FW	CAA AGC CAG AGC CAT TCA GAG
	IL-6 RV	GCC ATT CCG TCT GTG ACT CCA GTT TCT CC
	IL-10 FW	GGT TGC CAA GCC TTA TCA GA
	IL-10 RV	GCT GCA TTC TGA GGG TCT TC
	IL-12p40 FW	GAC ACG ACC TCC ACC AAA GT
	IL-12p40 RV	CAT TCT GGG ACT GGA CCC TA
	IL-17 FW²³	AGC TCC AGA GGC CCT CGG AC
	IL-17 RV²³	AGG ACC AGG ATC TCT TGC TG
	IFN-γ FW²⁴	CCA TGA ACG CTA CAC ACT GCA TC
	IFN-γ RV²⁴	GAA GTA GAA AGA GAC AAT CTG G
	TNF-α FW²³	GCT CCC CCA GAA GTC GGC G
	TNF-α RV²³	CTT GGT GGT TGG GTA CGA CA
Gastrin	Gastrin FW²⁵	GCC CTG GAA CCG CAA CA
Gastrin	Gastrin RV²⁵	TTC TTG GAC AGG TCT GCT TTG AA
H ⁺ /K ⁺ ATPase (parietal cells)	ATP4b FW	GGG GGT AAC CTT GAG ACC TGA TG
H ⁺ /K ⁺ ATPase (parietal cells)	ATP4b RV	AAG AAG TAC CTT TCC GAC GTG CAG
Reference genes	GAPDH FW²⁶	AAC GGC ACA GTC AAG GCT GAG AAC G
	GAPDH RV²⁶	CAA CAT ACT CGG CAC CGG CAT CG
	HPRT FW²⁷	CTC ATG GAC TGA TTA TGG ACA G
	HPRT RV²⁷	AGC TGA GAG ATC ATC TCC ACC AAT
	β-actin FW²⁸	CCA AGG CCA ACC GCG AGA TGA C
	β-actin RV²⁸	AGG GTA CAT GGT GGT GCC GCC AGA C

Primer pairs used for measuring the mRNA expression levels of IL-1β, IL-5, IL-6, IL-10, IL-12p40, IL-17, IFN-γ, TNF-α, gastrin and H⁺/K⁺ ATPase are shown. The housekeeping genes GAPDH, β-actin and HPRT were included as reference genes.

ISSN: 1297-9716