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Table 3 Effect of plasmid DNA concentration on transformation frequency.

From: Development of a self-replicating plasmid system for Mycoplasma hyopneumoniae

DNA quantity Average transformation frequency (transformants/CFU) [SE]
10 μg 5.6 × 10-3 [1.1 × 10-3]
5 μg 5.0 × 10-3 [1.4 × 10-3]
2 μg 3.3 × 10-4 [9.6 × 10-5]
1 μg 2.5 × 10-4 [3.7 × 10-5]
0 μg 0
  1. The effect of plasmid DNA concentration of transformation frequency was examined by transforming M. hyopneumoniae strain 232 with different quantities of pMHO-2 DNA. Average transformation frequencies (with standard error) of three transformations are shown.