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Table 2 Effect of electroporation voltage on transformation frequency.

From: Development of a self-replicating plasmid system for Mycoplasma hyopneumoniae

Electroporation voltage Average transformation frequency (transformants/CFU) [SE]
2.5 kV 5.4 × 10-6 [1.2 × 10-6]
2.0 kV 4.2 × 10-7 [3.9 × 10-7]
1.5 kV 0
1.0 kV 0
0 kV 0
  1. The effect of altering the electroporation voltage on transformation frequency was examined. M. hyopneumoniae strain 232 was transformed with pMHO-2 and the average transformation frequencies (with standard error) of three separate transformations are shown.