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Figure 2 | Veterinary Research

Figure 2

From: Development of a self-replicating plasmid system for Mycoplasma hyopneumoniae

Figure 2

Minimum oriC determination. The predicted oriC region of M. hyopneumoniae strain 232 is shown with the location of two putative DnaA boxes (black arrowheads) lying within AT-rich intergenic regions (grey boxes). The oriC region from plasmid pMHO (oriC-i) was reduced in a series of steps to produce five further plasmids (pOriC-ii, pOriC-iii, pOriC-iv, pOriC-iii/iv and pOriC-iv/v). M. hyopneumoniae strain 232 was transformed in triplicate with each plasmid and the mean transformation efficiencies (TF) are shown, expressed as the number of transformants per colony forming unit of mycoplasma transformed.

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