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Figure 6 | Veterinary Research

Figure 6

From: BAG3 protects Bovine Papillomavirus type 1-transformed equine fibroblasts against pro-death signals

Figure 6

BAG3 down-modulation sensitizes equine cell lines to PEITC-induced detachment. Control, scrRNA- and BAG3siRNA-treated cells were exposed to PEITC 42 h after transfection. Control and scrRNA-treated (scrRNA) EqS04b and E. Derm cells were exposed to 20 μM and 15 μM PEITC, respectively, while EqSO4b and E. Derm BAG3 silenced cells (siRNA) were exposed to 15 μM and 12 μM PEITC, respectively, in view of the lower number of cells (about 70-80% of control and scrRNA cells). (a) Phase contrast images of control (left panels), scrRNA-transfected (middle panels), and BAG3siRNA (siRNA)-transfected (right panels) cells were acquired at the times indicated (images from one experiment representative of three with similar results). (b) Percentages of adherent cells at 8 h following PEITC treatment in non-transfected (ctrl), scrRNA-transfected, and BAG3siRNA (siRNA)-transfected cells respect to their corresponding controls exposed to vehicle only. Data presented are the mean values ± S.E.M. from at least three experiments (*p < 0.05, **p < 0.001; scrRNA-transfected versus non-transfected or siRNA versus scrRNA).

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