Effect of BAG3 down-regulation on cell viability and morphology. (a) Number of viable cells in BAG3-silenced EqS04b and E. Derm cells at 48 h and 72 h following transfection. Data, expressed as a percentage of the number of scrRNA-transfected viable cells measured at the same times, are the mean values ± S.E.M. from at least three experiments (*p < 0.05, **p < 0.001 versus scrRNA-treated cells); (b) Phase contrast microscopy images of non-transfected (Ctrl), scrRNA- and BAG3siRNA (siRNA)-transfected EqS04b samples at 56 h after transfection. Images are from one analysis representative of at least three experiments with similar results.