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Table 1 Summary of immune gene expression in T. bryosalmonae infected rainbow trout kidney tissue

From: Immune gene expression profiling of Proliferative Kidney Disease in rainbow trout Oncorhynchus mykiss reveals a dominance of anti-inflammatory, antibody and T helper cell-like activities

Gene Grade 0 average cCq Kidney swelling grade
Grade 1 Grade 1-2 Grade 2 Grade 3 Pearson correlation
T. bryosalmonae 18S rDNA Not detectable 2105 . 69** 4266 . 94** 6652 . 34** 8034 . 26** . 755**
T. bryosalmonae RPL18   8013 . 47** 37233 . 66** 39385 . 81** 26654 . 46** . 740**
IL- 1β- 1 27.16 3 . 71* 2.24 2.10 2.31 -.116
TNF - α1 24.52 1.12 0.95 0.94 0.84 -.155
TNF - α2 26.60 0.97 2 . 19* 1.52 1.64 .106
IL - 6 34.12 7 . 44** 31 . 53** 23 . 59** 15 . 38** . 510**
IL - 11 29.62 31 . 74** 25 . 04** 14 . 13** 11 . 54** . 399**
M17 26.05 5 . 10** 6 . 28** 5 . 48** 2.60 .179
COX - 2A 24.69 2.15 1.68 1.75 1.07 -.161
COX - 2B 32.73 3.59 3 . 94* 3 . 10* 2.03 .164
MCSF - 1 22.92 1.18 0.77 0 . 49* 0 . 23** - . 658**
MCSF - 2 21.78 1.81 3 . 82* 2.77 1.41 -.076
MCSF - R1 27.65 1.05 2.48 1.01 0 . 57* - . 375*
MCSF - R2 21.77 2.15 2.70 2.65 1.22 -.001
iNOS 33.31 2.34 5 . 54* 2.43 1.28 -.035
Arginase - 1 15.20 1.05 0 . 50* 0 . 45* 0 . 23** - . 612**
Cathelicidin - 1 28.63 52 . 60** 136 . 21** 72 . 80** 57 . 81** . 530**
Cathelicidin - 2 29.58 9 . 00** 56 . 40** 94 . 90** 19 . 10** . 547**
Hepcidin - 1 25.76 5 . 36* 10 . 85* 22 . 37** 6 . 42** . 360*
LEAP - 2A 34.31 10 . 92** 12 . 87** 7 . 76** 6 . 84** .281
CD8α 25.31 5 . 37** 4 . 01** 5 . 46** 3 . 91* .238
CD8β 21.41 2 . 99* 3 . 05* 3 . 34* 2.33 .128
IL - 2Rβ 24.63 3 . 79** 5 . 19** 1.28 0.93 -.168
IgD H - secretory 21.39 0.98 1.02 0.89 0.92 -.095
IgM H - secretory 14.14 5 . 39* 3.38 8 . 80** 4 . 80** . 399**
IgT H - secretory 21.31 8 . 19* 24 . 68** 51 . 03** 68 . 62** . 732**
CD4 22.47 3 . 66** 4 . 28** 4 . 72** 3 . 56* .298
T - bet 24.94 7 . 00** 6 . 86** 7 . 06** 2 . 80** .203
IL - 2 28.68 3 . 68* 4 . 16** 2.16 2.54 .048
IFNγ 29.25 5 . 06** 15 . 40** 10 . 03** 8 . 49** . 500**
GATA3 25.00 4 . 64** 5 . 77** 3.63* 2.33 .115
IL - 4 / 13A 25.77 1.67 2.71 1.70 0.80 -.124
IL - 21 33.78 20 . 28** 42 . 62** 31 . 58** 11 . 91** . 506**
IL - 22 33.65 10 . 03* 6 . 67** 5 . 49* 4.48 .159
IL - 17A / F2a 28.27 10 . 84** 11 . 23** 2.64 3.24 .061
IL - 17C1 27.92 6 . 56** 2 . 74* 1.43 0.84 -.259
IL - 17C2 30.90 2.08 1.36 1.06 0.70 - . 349*
IL - 17D 31.72 4 . 95** 5 . 73** 3 . 78** 3 . 56* .259
FOXP3A 26.19 2.96 5 . 15* 4.75 2.52 .042
FOXP3B 27.03 4 . 02** 3 . 87** 3 . 13** 2.11 .109
TGF - β1a 22.87 3 . 34* 3 . 00** 2.02 1.59 -.015
nIL - 1F 24.76 3 . 14* 3 . 32** 2 . 81* 1.97 .136
IL - 10A 26.26 14 . 68** 18 . 21** 25 . 24** 9 . 51** . 414**
SOCS - 3 22.74 3 . 44* 8 . 38** 10 . 24** 5 . 90** . 472**
  1. The average base-line Cq (cCq) in control (grade 0) fish is provided for each gene studied with the exception of parasite genes, which were undetectable in control fish. Immune gene expression at each stage of clinical PKD (grade 1 to 3) was initially normalized to trout EF-1α and subsequently expressed as fold change relative to expression levels in control fish. Pearson correlation r coefficients are given relative to kidney swelling grade. Significant differences (2-tailed) between control and infection groups are shown in bold. * P < 0.05, ** P < 0.01.