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Table 1 Summary of immune gene expression in T. bryosalmonae infected rainbow trout kidney tissue

From: Immune gene expression profiling of Proliferative Kidney Disease in rainbow trout Oncorhynchus mykiss reveals a dominance of anti-inflammatory, antibody and T helper cell-like activities

Gene

Grade 0 average cCq

Kidney swelling grade

Grade 1

Grade 1-2

Grade 2

Grade 3

Pearson correlation

T. bryosalmonae 18S rDNA

Not detectable

2105 . 69**

4266 . 94**

6652 . 34**

8034 . 26**

. 755**

T. bryosalmonae RPL18

 

8013 . 47**

37233 . 66**

39385 . 81**

26654 . 46**

. 740**

IL- 1β- 1

27.16

3 . 71*

2.24

2.10

2.31

-.116

TNF - α1

24.52

1.12

0.95

0.94

0.84

-.155

TNF - α2

26.60

0.97

2 . 19*

1.52

1.64

.106

IL - 6

34.12

7 . 44**

31 . 53**

23 . 59**

15 . 38**

. 510**

IL - 11

29.62

31 . 74**

25 . 04**

14 . 13**

11 . 54**

. 399**

M17

26.05

5 . 10**

6 . 28**

5 . 48**

2.60

.179

COX - 2A

24.69

2.15

1.68

1.75

1.07

-.161

COX - 2B

32.73

3.59

3 . 94*

3 . 10*

2.03

.164

MCSF - 1

22.92

1.18

0.77

0 . 49*

0 . 23**

- . 658**

MCSF - 2

21.78

1.81

3 . 82*

2.77

1.41

-.076

MCSF - R1

27.65

1.05

2.48

1.01

0 . 57*

- . 375*

MCSF - R2

21.77

2.15

2.70

2.65

1.22

-.001

iNOS

33.31

2.34

5 . 54*

2.43

1.28

-.035

Arginase - 1

15.20

1.05

0 . 50*

0 . 45*

0 . 23**

- . 612**

Cathelicidin - 1

28.63

52 . 60**

136 . 21**

72 . 80**

57 . 81**

. 530**

Cathelicidin - 2

29.58

9 . 00**

56 . 40**

94 . 90**

19 . 10**

. 547**

Hepcidin - 1

25.76

5 . 36*

10 . 85*

22 . 37**

6 . 42**

. 360*

LEAP - 2A

34.31

10 . 92**

12 . 87**

7 . 76**

6 . 84**

.281

CD8α

25.31

5 . 37**

4 . 01**

5 . 46**

3 . 91*

.238

CD8β

21.41

2 . 99*

3 . 05*

3 . 34*

2.33

.128

IL - 2Rβ

24.63

3 . 79**

5 . 19**

1.28

0.93

-.168

IgD H - secretory

21.39

0.98

1.02

0.89

0.92

-.095

IgM H - secretory

14.14

5 . 39*

3.38

8 . 80**

4 . 80**

. 399**

IgT H - secretory

21.31

8 . 19*

24 . 68**

51 . 03**

68 . 62**

. 732**

CD4

22.47

3 . 66**

4 . 28**

4 . 72**

3 . 56*

.298

T - bet

24.94

7 . 00**

6 . 86**

7 . 06**

2 . 80**

.203

IL - 2

28.68

3 . 68*

4 . 16**

2.16

2.54

.048

IFNγ

29.25

5 . 06**

15 . 40**

10 . 03**

8 . 49**

. 500**

GATA3

25.00

4 . 64**

5 . 77**

3.63*

2.33

.115

IL - 4 / 13A

25.77

1.67

2.71

1.70

0.80

-.124

IL - 21

33.78

20 . 28**

42 . 62**

31 . 58**

11 . 91**

. 506**

IL - 22

33.65

10 . 03*

6 . 67**

5 . 49*

4.48

.159

IL - 17A / F2a

28.27

10 . 84**

11 . 23**

2.64

3.24

.061

IL - 17C1

27.92

6 . 56**

2 . 74*

1.43

0.84

-.259

IL - 17C2

30.90

2.08

1.36

1.06

0.70

- . 349*

IL - 17D

31.72

4 . 95**

5 . 73**

3 . 78**

3 . 56*

.259

FOXP3A

26.19

2.96

5 . 15*

4.75

2.52

.042

FOXP3B

27.03

4 . 02**

3 . 87**

3 . 13**

2.11

.109

TGF - β1a

22.87

3 . 34*

3 . 00**

2.02

1.59

-.015

nIL - 1F

24.76

3 . 14*

3 . 32**

2 . 81*

1.97

.136

IL - 10A

26.26

14 . 68**

18 . 21**

25 . 24**

9 . 51**

. 414**

SOCS - 3

22.74

3 . 44*

8 . 38**

10 . 24**

5 . 90**

. 472**

  1. The average base-line Cq (cCq) in control (grade 0) fish is provided for each gene studied with the exception of parasite genes, which were undetectable in control fish. Immune gene expression at each stage of clinical PKD (grade 1 to 3) was initially normalized to trout EF-1α and subsequently expressed as fold change relative to expression levels in control fish. Pearson correlation r coefficients are given relative to kidney swelling grade. Significant differences (2-tailed) between control and infection groups are shown in bold. * P < 0.05, ** P < 0.01.
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Veterinary Research

ISSN: 1297-9716