Western blot of serum from broiler chickens immunized with rNetB. Recombinant NetB was electrophoresied on a 4%-12% SDS-PAGE and transferred onto a PVDF membrane. The blot was developed with an ECL Western blotting kit and results recorded on autoradiographic film. SeeBlue Plus2 prestained marker (Invitrogen) was used as a size marker (kDa). Birds were immunized with 50 μg of rNetB with CSIRO Triple adjuvant (days 7 and 17) and serum collected on day 28. A, probed with sera from birds vaccinated with adjuvant only; N, probed with sera from birds vaccinated with rNetB in adjuvant.