Table 2 CyHV-3 and host proteins identified by 2D-LC MS/MS in the supernatant of CyHV-3 infected CCB cells.
Database | Accession number | Description | Predicted MW (kDa) | Mascot score | No. of matching spectra | emPAI |
|---|---|---|---|---|---|---|
CyHV-3 proteins | ||||||
Cyprinivirus | gi|129560530 | ORF12, TNF receptor superfamily homologue | 19.2 | 671 | 14 | 1.49 |
gi|129560652 | ORF134, Interleukin 10 homologue | 14.8 | 304 | 6 | 1.02 | |
gi|84181525 | ORF116, predicted membrane glycoprotein | 30.4 | 185 | 4 | 0.26 | |
gi|84181523 | ORF119, putative uncharacterized protein containing an hydrophobic region | 15.5 | 94 | 2 | 0.25 | |
gi|129560569 | ORF52, predicted membrane glycoprotein | 39.2 | 44 | 1 | 0.10 | |
Host proteins (species origin) | ||||||
Bony vertebrates | gi|122891218 | Novel protein (zgc:103659) (Danio rerio) | 51.7 | 410 | 13 | 0.42 |
gi|136429 | Trypsin (Sus scrofa) | 25.1 | 281 | 9 | 0.53 | |
gi|297262447 | Predicted keratin, type II cytoskeletal 1-like isoform 6 (Macaca mulatta) | 65.3 | 268 | 3 | 0.12 | |
gi|37590349 | Enolase 1, alpha (D. rerio) | 47.4 | 255 | 8 | 0.35 | |
gi|326670662 | Predicted collagen alpha-3(VI) chain-like (D. rerio) | 11.3 | 227 | 8 | 0.17 | |
gi|51949771 | Fibronectin 1b (D. rerio) | 279.3 | 213 | 8 | 0.08 | |
gi|52218922 | Pigment epithelium-derived factor precursor (D. rerio) | 45.0 | 158 | 3 | 0.17 | |
gi|416696 | Beta-2-microglobulin (Cyprinus carpio) | 13.5 | 152 | 8 | 1.79 | |
gi|1351907 | Serum albumin (Bos taurus) | 71.2 | 149 | 11 | 0.36 | |
gi|395744345 | Predicted keratin, type II cytoskeletal 1 (Pongo abelii) | 25.8 | 139 | 3 | 0.15 | |
gi|229552 | Albumin (B. taurus) | 68.1 | 136 | 10 | 0.38 | |
gi|63102189 | Pgd protein (D. rerio) | 53.7 | 134 | 4 | 0.14 | |
gi|15718387 | Gelatinase (Paralichthys olivaceus) | 75.5 | 125 | 5 | 0.15 | |
gi|1703244 | Fructose-bisphosphate aldolase C (Carassius auratus) | 39.8 | 124 | 4 | 0.20 | |
gi|169154447 | Fibronectin 1 (D. rerio) | 275.6 | 117 | 5 | 0.05 | |
gi|15149946 | Procollagen type I alpha 1 chain (D. rerio) | 49.4 | 117 | 5 | 0.34 | |
gi|148726027 | Cadherin 11, osteoblast (D. rerio) | 88.9 | 112 | 5 | 0.13 | |
gi|4885063 | Fructose-bisphosphate aldolase C (Homo sapiens) | 39.8 | 107 | 2 | 0.20 | |
gi|28336 | Mutant beta-actin (beta'-actin) (H. sapiens) | 42.1 | 105 | 2 | 0.09 | |
gi|28317 | Unnamed protein product (H. sapiens) | 59.7 | 104 | 3 | 0.13 | |
gi|337758 | Pre-serum amyloid P component (H. sapiens) | 25.5 | 100 | 3 | 0.32 | |
gi|223582 | Histone H4 (H. sapiens) | 11.2 | 99 | 5 | 0.84 | |
gi|47971186 | Carp C1q-like molecule (C. carpio) | 20.3 | 98 | 2 | 0.19 | |
gi|223061 | Ubiquitin (Salmo sp.) | 8.5 | 92 | 4 | 0.31 | |
gi|27806751 | Alpha-2-HS-glycoprotein precursor (B. taurus) | 39.2 | 90 | 4 | 0.32 | |
gi|47086029 | Myristoylated alanine-rich C kinase substrate 2 (D. rerio) | 21.0 | 86 | 2 | 0.18 | |
gi|2133885 | N-cadherin precursor (D. rerio) | 87.4 | 80 | 3 | 0.09 | |
gi|18859555 | Wnt inhibitory factor 1 precursor (D. rerio) | 43.2 | 79 | 3 | 0.09 | |
gi|34595971 | Prion-like protein 1 (C. carpio) | 55.6 | 75 | 2 | 0.14 | |
gi|208609649 | Collagen type I alpha 3 (C. auratus) | 137.7 | 75 | 1 | 0.03 | |
gi|47085905 | 14-3-3 protein beta/alpha-B (D. rerio) | 27.5 | 75 | 3 | 0.47 | |
gi|6644111 | Nucleoside diphosphate kinase-Z1 (D. rerio) | 17.4 | 72 | 2 | 0.22 | |
gi|16974825 | Chain A, Solution Structure Of Calcium-Calmodulin N-Terminal Domain (H. sapiens) | 8.5 | 70 | 2 | 0.49 | |
gi|41152406 | FK506 binding protein 1A, 12kDa (D. rerio) | 11.8 | 69 | 3 | 1.39 | |
gi|189527793 | Predicted neuroblast differentiation-associated protein AHNAK (D. rerio) | 642.1 | 65 | 2 | 0.01 | |
gi|37492 | Alpha-tubulin (H. sapiens) | 50.8 | 65 | 2 | 0.07 | |
gi|33989505 | Tissue inhibitor of metalloproteinase 2b (D. rerio) | 24.7 | 64 | 2 | 0.15 | |
gi|8176557 | Heart fatty acid binding protein (Anguilla japonica) | 15.3 | 61 | 1 | 0.26 | |
gi|37181 | Tissue inhibitor of metalloproteinases, Type-2 (H. sapiens) | 21.4 | 59 | 2 | 0.18 | |
gi|437972 | Fibrillin-2 (H. sapiens) | 334.8 | 59 | 1 | 0.01 | |
gi|37367051 | Osteopontin (D. rerio) | 23.2 | 53 | 1 | 0.16 | |
gi|45544646 | Cold inducible RNA binding protein isoform 2 (D. rerio) | 19.2 | 52 | 2 | 0.20 | |
gi|51328294 | Fstl1b protein (D. rerio) | 39.6 | 50 | 1 | 0.09 | |
gi|82245450 | Triosephosphate isomerase B (D. rerio) | 27.1 | 50 | 1 | 0.14 | |
gi|34014734 | Clusterin (D. rerio) | 52.5 | 50 | 1 | 0.07 | |
gi|47228578 | Unnamed protein product (Tetraodon nigroviridis) | 77.5 | 49 | 1 | 0.05 |