|
Primers for PCR and RT-PCR
|
PCR
|
RT-PCR
| | |
|
CyHV-3 ORF3
|
ORF3InF
| |
•
|
TATGCCCACATGATGCTGTT
|
DQ657948
|
| |
ORF3InR
| |
•
|
CAGTCAGACCCTTCCTCTGC
| |
|
CyHV-3 ORF55
|
ORF55InF
|
•
| |
AGCGCTACACCGAAGAGTCC
| |
| |
ORF55stopR
|
•
| |
TCACAGGATAGATATGTTACAAG
| |
| |
ORF55ATGF
| |
•
|
ATGGCTATGCTGGAACTGG
| |
| |
ORF55InR
| |
•
|
GGCGCACCCAGTAGATTATG
| |
|
CyHV-3 ORF78
|
ORF78InF
| |
•
|
TGGACGACGAACACCCTTC
| |
| |
ORF78InR
| |
•
|
GGTAGAGGGTACAACCACG
| |
|
CyHV-3 ORF132
|
ORF132InF
| |
•
|
GGATCCGTTTTCTGGGTCTG
| |
| |
ORF132InR
| |
•
|
CTCAATCCCTCACCGACCTC
| |
|
CyHV-3 ORF133
|
ORF133InF
| |
•
|
GACGAGATCCCTATCCGCAG
| |
| |
ORF133InR
| |
•
|
GACCTCGGGTATGGTCGGTA
| |
|
CyHV-3 ORF134
|
ORF134stopF
| |
•
|
TCAATGTTTGCGCTTGGTTTTC
| |
| |
ORF134ATGR
| |
•
|
ATGTTCCTTGCAGTGCTAC
| |
| |
ORF134InF
|
•
| |
GGTTTCTCTTTGTAGTTTTCCG
| |
| |
ORF134InR
|
•
| |
CACCCCAACTTTTGAGACAAC
| |
| |
ORF134outseqF
|
•
| |
GTCAACATGGACGAGCGTGA
| |
| |
ORF134outseqR
|
•
| |
GTGGGGATATCAAACACGCA
| |
|
CyHV-3 ORF135
|
ORF135InF
| |
•
|
ACACCACCAACGAGACATGC
| |
| |
ORF135InR
| |
•
|
CTTTTCGGACCAGAAGACCG
| |
|
Carp β-actin
|
Actin-F
| |
•
|
ATGTACGTTGCCATCCAGGC
|
M24113
|
| |
Actin-R
| |
•
|
GCACCTGAACCTCTCATTGC
| |
|
Primers for amplification of recombination cassettes
|
|
H1-gal K-H2 cassette
|
134 gal K F
| | |
ATGTTCCTTGCAGTGCTACTAACCG
|
Warming et al. [34]
|
|
CGACCATCTTCTTCGAGGCTCGGGG
|
|
CCTGTTGACAATTAATCATCGGCA
|
| |
134 gal K R
| | |
TCAATGTTTGCGCTTGGTTTTCATG
| |
|
TTCTTGACGTCTTTTGCGACCAGGA
|
|
TCAGCACTGTCCTGCTCCTT
|
|
H1-ORF134-H2
|
H1F
| | |
GCTCATCAATCGCAGCAGCA
|
DQ657948
|
|
cassette
|
| |
H2R
| | |
CAAGCCATTATCCTGTTGGG
| |
|
Primers and probes for real-time TaqMan PCR quantification of CyHV-3 genome
|
|
CyHV-3 ORF89
|
KHV-86F
| | |
GACGCCGGAGACCTTGTG
|
AF411803
|
| |
KHV-163R
| | |
CGGGTTCTTATTTTTGTCCTTGTT
| |
| |
KHV-109P
| | |
(6FAM)CTTCCTCTGCTCGGCGAGCACG(BHQ1)
| |
|
Carp glucokinase
|
CgGluc-162F
| | |
ACTGCGAGTGGAGACACATGAT
|
AF053332
|
| |
CgGluc-230R
| | |
TCAGGTGTGGAGCGGACAT
| |
| |
CgGluc-185P
| | |
(6FAM)AAGCCAGTGTCAAAATGCTGCCCACT(BHQ1)
| |
|
Primers for RT-qPCR analysis of carp gene expression
|
|
40S
|
40S-F
| | |
CCGTGGGTGACATCGTTACA
|
AB012087
|
| |
40S-R
| | |
TCAGGACATTGAACCTCACTGTCT
| |
|
IL-1β
|
IL-1β-F
| | |
AAGGAGGCCAGTGGCTCTGT
|
AJ245635
|
| |
IL-1β-R
| | |
CCTGAAGAAGAGGAGGCTGTCA
| |
|
TNF-α1 and 2
|
TNF-α1 and 2-F
| | |
GCTGTCTGCTTCACGCTCAA
|
AJ311800
|
| |
TNF-α1 and 2-R
| | |
CCTTGGAAGTGACATTTGCTTTT
| |
|
CXCa
|
CXCa-F
| | |
CTGGGATTCCTGACCATTGGT
|
AJ421443
|
| |
CXCa-R
| | |
GTTGGCTCTCTGTTTCAATGCA
| |
|
IL-10
|
IL-10-F
| | |
CGCCAGCATAAAGAACTCGT
|
AB110780
|
| |
IL-10-R
| | |
TGCCAAATACTGCTCGATGT
| |
|
IFNγ-2
|
IFNγ-2-F
| | |
TCTTGAGGAACCTGAGCAGAA
|
AM168523
|
| |
IFNγ-2-R
| | |
TGTGCAAGTCTTTCCTTTGTAG
| |
|
IL-6
|
IL-6M17-F
| | |
CACATTGCTGTGAGGGTGAA
|
AY102633
|
| |
IL-6M17-R
| | |
GCATCCATAGGCTTTCTGCT
| |
