Viral load in gill and kidney. Common carp (n = 36 fish per tank), with an average weight of 10.7 g ± 3.2 g (mean ± SD), were inoculated by immersion for 2 h in water containing 100 PFU/mL of the indicated CyHV-3 strains. At different times post-inoculation, six infected fish were randomly selected per tank, euthanized and dissected. Six mock-infected fish were used as negative controls. Gill, kidney and spleen were harvested and stored in RNAlater® at −80 °C. DNA was extracted from gill (panel A) and kidney (panel B) and analyzed by real-time TaqMan PCR for quantification of viral genome copies. The results are expressed as the means ± SD of the data observed for the 6 fish analyzed per time point. Spleen were treated for quantification of carp gene expression by RT-qPCR (see Figure 11).