Skip to main content
Figure 3 | Veterinary Research

Figure 3

From: The molecular basis for recognition of bacterial ligands at equine TLR2, TLR1 and TLR6

Figure 3

Responses of human and equine TLR2/6 to Pam2CSK4. SW620 cells were transiently transfected with hTLR2 and hTLR6 or eqTLR2 and eqTLR6, together with the cognate species’ CD14 and reporter constructs NF-κB-luc and Renilla luciferase. Cells were stimulated 24 hours later with increasing doses of Pam2CSK4. Cells were stimulated for six hours, lysed, and analysed for luciferase activity. Data are from a representative experiment and expressed as triplicate mean ± SEM for that experiment. Pam2CSK4 stimulated human and equine TLR2/6 dose-dependently. Data were normalised for calculation of EC50, using medium alone as 0% and maxima for each curve (raw data) as 100%. EC50 values were not significantly different for human and equine receptors.

Back to article page