Table 2 Primers targeting the prophage-associated genomic island of porcine L. intracellularis isolates and the two flanking genes
Gene | Gene product | Entrez gene ID | Primers (5′ → 3′) |
|---|---|---|---|
LI0172* | Hypothetical protein | 4059866 | ATTGATGCTCCTGTCCCACG |
| Â | ACCACATGGTGGATTCGTCC | ||
LI0173 | Prophage DLP12 integrase | 4059867 | CGTCGTATTCTGCGCTTTGG |
| Â | ATCATCAGCTACACGAGCGG | ||
LI0174 | Hypothetical protein | 4059868 | CAGGAAGATGCTGTGTGGCT |
| Â | ATTCGCTTTCGCAATACGGC | ||
LI0175 | Hypothetical protein | 4059869 | CCCACGGACGAAGACTTTGA |
| Â | TCAGCTTTCGGGCATGGATT | ||
LI0176 | Hypothetical protein | 4059870 | ACAGACCTCTATGCTCCCGT |
| Â | TCAGCGTCTTGGGGCTTTAG | ||
LI0177 | Hypothetical protein | 4059871 | ACACCACCATTACCACTGCT |
| Â | ACACCACCATTACCACTGCT | ||
LI0178 | Hypothetical protein | 4059872 | TTCCTCCTGCGTGTCGTAAC |
| Â | ATTTCTCCCTGGCTCTGCAC | ||
LI0179 | Ribosomal protection tetracycline resistance protein | 4059813 | CGTCAGCAAAGCGGAAACAA |
| Â | AAACGGCCTTGGCATTCAAC | ||
LI0180 | Hypothetical protein | 4059873 | GAACCGGTGAGCCAAGTGTA |
| Â | TTCCTTCGGGAGTCGAGGAT | ||
LI0181 | Endonuclease I | 4059874 | AGGCTAAGCGCATACTGCAA |
| Â | CAGCATTGACAGACCCGACT | ||
LI0182 | Recombination protein-phage associated | 4059875 | TGGATTTCCAGCACAGCCAT |
| Â | TGAACCGTCCTGAAGCTCAC | ||
LI0183 | Hypothetical protein | 4060170 | CTCTCGACGCATCTTCCCTC |
| Â | TCCATTCCGCCGTCATGAAA | ||
LI0184 | Hypothetical protein | 4060171 | TTGGACTGGCTCTTACGCAG |
| Â | GATGAAGCCCACGTCAGGAA | ||
LI0185 | MoxR-like ATPases | 4060172 | GTCATGCGTCAGAACATCGC |
| Â | CCTGTTTGGAGAGAGGCTGG | ||
LI0186 | Hypothetical protein | 4060173 | CCTTCCTGGGCCAACATCAT |
| Â | CACGCTTGGGCATATTTCCG | ||
LI0187 | Hypothetical protein | 4060174 | ATCGGTTCTTCGGATACCGC |
| Â | GAATCCTGCGTAGATCGGCA | ||
LI0188* | ATP-dependent Zn proteases (ftsH) | 4059755 | GAGCTGTAGCTGGTGAAGCA |
| Â | Â | Â | ACGTGCAACAAGTGCATGTC |