The clinical outcome of cattle challenged with FMDV. Cattle treated with one dose, 1/3 dose, 1/9 dose of FMD VLP and healthy cattle as control were challenged with 10 000 ID50 of FMDV Asia1/Jiangsu/China/2005 at 21 dpi. The animals were carefully examined in the muzzle, inside the mouth and feet every day for 10 days after challenge with the homogenous virus. A clinical score of 0 to 5 (right Y axis) was assigned to describe the severity of the disease, with a maximal score of 5: a score of 0 was assigned when one or more vesicular lesions were detected at the site of injection on the tongue; a score of 1 was assigned when one or more vesicular lesions were detected in the mouth other than the tongue; a score of 2 was assigned when one or more vesicular lesions were detected in one foot, a score of 3 was assigned if two feet had vesicular lesions, a score of 4 was assigned if three feet had vesicular lesions, a score of 5 was assigned if four feet had vesicular lesions. Animals showing viremia were selected and listed. The maximum clinical score (bars) is 5. Viremia (left Y axis) determined by infectivity and viral RNA level are expressed as log10 PFU/mL and log10 copies/mL respectively.