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Figure 3 | Veterinary Research

Figure 3

From: Transgenically mediated shRNAs targeting conserved regions of foot-and-mouth disease virus provide heritable resistance in porcine cell lines and suckling mice

Figure 3

Establishment of transgenic monoclonal colonies and the development of MTCL. The shRNA-expressing plasmids PB-EN3D2B or PB-ENlacZNH21 were integrated into the genome of IBRS-2 cells using the piggyBac transposon system. Monoclonal colonies with both G418 resistance and EGFP expression were selected (A, magnification is 100×). The colonies were then cultured to generate cell lines. All of the cells expressed EGFP (B, magnification is 200×). The images were adjusted for contrast and dpi (dot per inch) to improve their appearance.

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Veterinary Research

ISSN: 1297-9716