Figure 5

Effect of IFN-β alone or in combination with SaS on the expression of IFN-inducible genes by bMEC. a) Response of bMEC to increasing concentrations of recombinant human IFN-β. After 3 or 6 h of incubation with IFN-β, the relative expression of the Cxcl10 and Isg15 genes was determined by RT-qPCR. b) Effect of adding IFN-β (10 ng/mL) to SaS after 3 h of exposure to SaS, and comparison with the effect on Cxcl10 and Isg15 expression of SaS and LPs alone, measured 3 h after addition of IFN-β. c) Effect of adding IFN-β as in b) on the expression of Ccl5, Ccl2 and Nos2a. * P < 0.05, relative to Medium; $ P < 0.05, LPS versus SaS.