Figure 1

Activity of crude LPS (LPS Sigma) and SaS (SaS N305) on HEK 293 cells transfected with human TLR2, TLR4, TLR5, Nod1 or Nod2. a) Pure LPS (ULPS) and purified LTA were used as agonists of TLR4 and TLR2, respectively. The response of HEK Blue cells (InvivoGen) was measured spectrophotometrically (OD 630 nm). b) Flagellin, C12-iE-DAP and MDP were used as agonists of TLR5, Nod1 and Nod2, respectively. The response of HEK 293 cells was evaluated through secretion of CXCL8, measured by ELISA.