Quantity of virulent fowl adenovirus serotype 1 correlates with clinical signs, macroscopical and pathohistological lesions in gizzards following experimental induction of gizzard erosion in broilers

Table 1 Pathological changes in the gizzard

		Macroscopical lesions		Histological lesions
Group	dpi	Koilin layer	Gizzard mucosa	Inclusion bodies in glandular epithelium	*In-situ* hybridization
SPFB	3	0/5 (0.0)^a	0/5 (0.0)	0/5 (0.0)	0/5
	7	5/5 (1.8)	5/5 (1.8)	5/5 (2.6)	5/5
	10	5/5 (2.4)	5/5 (1.0)	1/5 (0.2)	1/5
	14	5/5 (1.8)	2/5 (0.4)	0/5 (0.0)	NE^b
	17	4/5 (0.8)	3/5 (0.6)	0/5 (0.0)	NE
CB	3	0/5 (0.0)	0/5 (0.0)	0/5 (0.0)	0/5
	7	3/5 (0.6)	3/5 (0.8)	4/5 (1.6)	4/5
	10	4/5 (1.2)	5/5 (1.6)	5/5 (2.0)	5/5
	14	5/5 (2.2)	4/5 (0.8)	0/5 (0.0)	NE
	17	5/5 (1.6)	4/5 (0.8)	0/5 (0.0)	NE

Specific-pathogen-free (SPFB) and commercial (CB) broilers orally infected with virulent FAdV-1 at 3, 7, 10, 14 and 17 days post infection (dpi).
^a No. birds positive/no. birds examined (mean lesion scores in parenthesis; no lesion = 0, mild lesions = 1, moderate lesions = 2 and severe lesions = 3).
^b NE – not examined.

ISSN: 1297-9716