Figure 3

Graphical illustration of the viral load. Viral genome copies per reaction (log10) from a) gizzard, b) liver and c) cloacal swabs calculated by real-time PCR from specific-pathogen-free (SPF) and commercial broilers orally infected with virulent FAdV-1 at 3, 7, 10, 14 and 17 days post infection. Asterisks indicate some samples that turned out positive with specific melting curve analysis but viral genome copies per reaction could not be calculated successfully.