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Table 3 Antibody array results

From: Salmonella enterica Typhimurium infection causes metabolic changes in chicken muscle involving AMPK, fatty acid and insulin/mTOR signaling

Protein name (phosphorylated residue) Fold change antibody array P-value Orthology Fold change peptide array
4E-BP1 (Phospho-Ser65) −1.43712 0.00137 93% N/A
4E-BP1 (Phospho-Thr70) −1.36207 0.013516 86% N/A
AKT (Phospho-Ser473) 1.579649 0.002667 100% 1.61614
AKT (Phospho-Thr308) 1.898775 0.003797 100% 1.31019
AKT1 (Phospho-Ser124) −1.47999 0.000402 100% N/A
AKT1 (Phospho-Thr450) −1.5584 0.000476 100% N/A
AKT1S1 (Phospho-Thr246) −1.79817 2.22E-05 0% N/A
AMPK1 (Phospho-Thr174) −1.22623 0.0154 100% −1.09134
AMPKbeta1 (Phospho-Ser182) −1.34017 0.029026 93% −1.10925
BAD (Phospho-Ser112) 1.40376 0.012781 78% N/A
BAD (Phospho-Ser134) 1.380989 0.020394 0% N/A
BAD (Phospho-Ser136) 1.29749 0.039652 0% N/A
eIF4E (Phospho-Ser209) −1.33384 0.030631 100% N/A
ERK3 (Phospho-Ser189) 1.210088 0.019255 100% 4.01876
GSK3 beta (Phospho-Ser9) 2.974451 0.002007 100% 1.12689
IR (Phospho-Tyr1355) 3.187483 2.62E-05 53% N/A
IR (Phospho-Tyr1361) 1.685564 0.012336 93% N/A
P70S6K (Phospho-Ser371) −1.73418 0.000111 100% N/A
P70S6K (Phospho-Ser418) −1.58607 0.000109 100% N/A
P70S6K (Phospho-Ser424) −1.39268 0.037721 100% N/A
P70S6K (Phospho-Thr229) −1.44379 0.000416 100% N/A
P70S6K (Phospho-Thr389) −1.84347 0.009943 100% −1.12207
P70S6K (Phospho-Thr421) −1.22221 0.013127 100% N/A
P70S6k-beta (Phospho-Ser423) 1.60054 0.000107 100% N/A
P90RSK (Phospho-Ser380) 1.255345 0.04783 100% −1.12470
PDK1 (Phospho-Ser241) 1.632697 0.002201 93% −1.01557
PI3-kinase p85-alpha (Phospho-Tyr607) −1.3092 0.038374 87% −1.34043
PIP5K (Phospho-Ser307) −1.2407 0.026195 100% N/A
PTEN (Phospho-Ser370) 1.491624 0.002252 100% N/A
PTEN (Phospho-Ser380) 1.533421 0.005982 100% 1.12240
  1. Statistically significant (p < 0.05) phosphospecific antibody array analysis results of Salmonella Typhimurium infected muscle samples. One week post-infection samples were compared to 24 h post-infection samples to find changes in infected muscle over time. Antibodies due to being bound to phosphorylated protein had a statistically significant difference in fluorescent signal are shown. Fold Change Antibody Array is the change in fluorescent signal when comparing the 1 week and 24 h infected samples as indicated by the antibody array. Orthology indicates the % similarity between human and chicken at the 15 amino acid region flanking the phosphorylation residue. Fold Change Peptide Array is the change in fluorescent signal when comparing the 1 week and 24 h infected samples as indicated by the peptide array. N/A indicates the exact phosphorylation target residue on the antibody array was not present on the peptide array.