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Table 3 Antibody array results

From: Salmonella enterica Typhimurium infection causes metabolic changes in chicken muscle involving AMPK, fatty acid and insulin/mTOR signaling

Protein name (phosphorylated residue)

Fold change antibody array

P-value

Orthology

Fold change peptide array

4E-BP1 (Phospho-Ser65)

−1.43712

0.00137

93%

N/A

4E-BP1 (Phospho-Thr70)

−1.36207

0.013516

86%

N/A

AKT (Phospho-Ser473)

1.579649

0.002667

100%

1.61614

AKT (Phospho-Thr308)

1.898775

0.003797

100%

1.31019

AKT1 (Phospho-Ser124)

−1.47999

0.000402

100%

N/A

AKT1 (Phospho-Thr450)

−1.5584

0.000476

100%

N/A

AKT1S1 (Phospho-Thr246)

−1.79817

2.22E-05

0%

N/A

AMPK1 (Phospho-Thr174)

−1.22623

0.0154

100%

−1.09134

AMPKbeta1 (Phospho-Ser182)

−1.34017

0.029026

93%

−1.10925

BAD (Phospho-Ser112)

1.40376

0.012781

78%

N/A

BAD (Phospho-Ser134)

1.380989

0.020394

0%

N/A

BAD (Phospho-Ser136)

1.29749

0.039652

0%

N/A

eIF4E (Phospho-Ser209)

−1.33384

0.030631

100%

N/A

ERK3 (Phospho-Ser189)

1.210088

0.019255

100%

4.01876

GSK3 beta (Phospho-Ser9)

2.974451

0.002007

100%

1.12689

IR (Phospho-Tyr1355)

3.187483

2.62E-05

53%

N/A

IR (Phospho-Tyr1361)

1.685564

0.012336

93%

N/A

P70S6K (Phospho-Ser371)

−1.73418

0.000111

100%

N/A

P70S6K (Phospho-Ser418)

−1.58607

0.000109

100%

N/A

P70S6K (Phospho-Ser424)

−1.39268

0.037721

100%

N/A

P70S6K (Phospho-Thr229)

−1.44379

0.000416

100%

N/A

P70S6K (Phospho-Thr389)

−1.84347

0.009943

100%

−1.12207

P70S6K (Phospho-Thr421)

−1.22221

0.013127

100%

N/A

P70S6k-beta (Phospho-Ser423)

1.60054

0.000107

100%

N/A

P90RSK (Phospho-Ser380)

1.255345

0.04783

100%

−1.12470

PDK1 (Phospho-Ser241)

1.632697

0.002201

93%

−1.01557

PI3-kinase p85-alpha (Phospho-Tyr607)

−1.3092

0.038374

87%

−1.34043

PIP5K (Phospho-Ser307)

−1.2407

0.026195

100%

N/A

PTEN (Phospho-Ser370)

1.491624

0.002252

100%

N/A

PTEN (Phospho-Ser380)

1.533421

0.005982

100%

1.12240

  1. Statistically significant (p < 0.05) phosphospecific antibody array analysis results of Salmonella Typhimurium infected muscle samples. One week post-infection samples were compared to 24 h post-infection samples to find changes in infected muscle over time. Antibodies due to being bound to phosphorylated protein had a statistically significant difference in fluorescent signal are shown. Fold Change Antibody Array is the change in fluorescent signal when comparing the 1 week and 24 h infected samples as indicated by the antibody array. Orthology indicates the % similarity between human and chicken at the 15 amino acid region flanking the phosphorylation residue. Fold Change Peptide Array is the change in fluorescent signal when comparing the 1 week and 24 h infected samples as indicated by the peptide array. N/A indicates the exact phosphorylation target residue on the antibody array was not present on the peptide array.
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Veterinary Research

ISSN: 1297-9716