Figure 2From: Nuclear translocation and accumulation of glyceraldehyde-3-phosphate dehydrogenase involved in diclazuril-induced apoptosis in Eimeria tenella (E. tenella)Nuclear translocation and accumulation of endogenous GAPDH after diclazuril treatment. A difference in subcellular localization of endogenous GAPDH was observed in second-generation merozoites between the control group and the Treatment group. The distribution of endogenous GAPDH was detected by fluorescence microscopy; the GAPDH immunostaining was almost undetectable in the nuclei of second-generation merozoites of the control group (A). In contrast, the considerable GAPDH immunostaining (green) appeared to be in the nuclei of second-generation merozoites of the treatment group (B). DAPI staining (blue) to detect parasite nuclei. Merge is GAPDH immunostaining/DAPI staining overlay. Arrows show nuclei localization.Back to article page