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Table 1 Mass Spectrometry data for protein spots from DIGE analysis of BALF sampled within 2–3 days of arrival to the feedlot, for which spot densities were greater in calves that remained healthy compared to those that later developed pneumonia

From: Increased annexin A1 and A2 levels in bronchoalveolar lavage fluid are associated with resistance to respiratory disease in beef calves

Spot #

Protein identified

Acc #a

Cov %b

Probc

Pep matchd

AAe

MWf(kDa)

PIg

Fold increase

P value

1167

Macrophage capping protein

gi30466254

20

100

6

71/349

40/39

6/5.3

2.01

0.020

1207

Annexin A1

gi74

62

100

21

213/346

38/39

6.0/6.4

1.94

0.027

1227

Annexin A1

gi74

55

100

18

190/346

38/39

6.2/6.4

2.02

0.030

1231

Annexin A2

gi27807289

23

100

8

77/339

37/39

6.4/6.9

2.33

0.036

1249

Annexin A2

gi27807289

42

100

13

143/339

37/39

6.2/6.9

1.94

0.007

1258

Annexin A2

gi27807289

51

100

17

172/339

37/39

6.6/6.9

2.24

0.018

1259

Dihydrodiol dehydrogenase 3

gi3079344

30

100

12

98/323

37/37

6.8/7.6

2.69

0.018

1263

Dihydrodiol dehydrogenase 3

gi3079344

41

100

11

132/323

37/37

7/7.6

2.91

0.022

1685

Peroxiredoxin I

gi66773956

41

100

9

82/199

20/22

7.3/8.6

2.69

0.004

1713

Peroxiredoxin I

gi66773956

45

100

10

90/199

20/22

7.4/8.6

2.31

0.038

1728

Calcyphosin

gi115497428

38

100

7

72/189

21/21

4.8/4.8

2.62

0.008

1913

Superoxide dismutase, chain B

gi12084767

23

100

3

35/152

15/16

5.8/5.7

1.89

0.022

  1. a Acc #: Accession number. The number associated with a protein in a FASTA database, that Scaffold software uses to link to information about that protein on the NCBI website.
  2. b Cov %: Percent coverage; percentage of the predicted peptide identified by the mass spectrometry data.
  3. c Prob: The calculated probability of that a given protein has been identified correctly.
  4. d Pep match: Number of unique peptides on which the protein identification was based.
  5. e AA: Number of amino acids identified / total number of amino acids in the protein.
  6. f MW: Observed / theoretical molecular weight of the protein in kilodaltons.
  7. g pI: Observed / theoretical isoelectric point of the protein.