Figure 3

Western blot of BALF samples labeled with antibody to annexin A2. Lane 1: synthetic annexin A1 peptide, 2: synthetic annexin A2 peptide (positive control), 3: molecular weight ladder, 4–6: samples from calves that remained healthy; 7–9: samples from calves that later developed pneumonia, 10: negative control. The two bands indicate that there are two isoforms of the annexin A2 present in BALF, and the density of the bands is higher in the calves that remained healthy compared to the calves that later developed pneumonia.