Skip to main content
Figure 5 | Veterinary Research

Figure 5

From: Bovine HEXIM1 inhibits bovine immunodeficiency virus replication through regulating BTat-mediated transactivation

Figure 5

RNAi Knockdown of BHEXIM1 in FBL cells enhances BIV replication. (A) FBL cells were stably transfected with either BHEXIM1-specific (shBHEXIM1) or control (shCON) shRNA oligonuceotides. Cellular RNA was extracted and subjected to real-time PCR to examine the amount of BHEXIM1 and GAPDH. (B) BHEXIM1-knockdown (shBHEXIM1) and control (shCON) FBL cells (5 × 105/well) were infected with 5 × 103 TCID50 BIV. The mRNA level of BIV CA was examined by real-time PCR at 24 h post-infection. (C) BHEXIM1-knockdown (shBHEXIM1) and control (shCON) FBL cells (5 × 104/well) were infected with 5 × 102 TCID50 BIV. At 24 h post-infection, BIVL (1 × 105/well) was added and the luciferase activity was assayed after 48 h co-culture. Error bars represent the SD of the means from three independent experiments.

Back to article page