Specificity
|
Antigen or cell detected
|
mAb/pAb
|
Dilution
|
Pre-treatment
|
Source
|
---|
Anti-human MΦs
|
Monocytes and MΦs
|
mAb
|
1:100
|
Proteasea
|
Serotec
|
| |
(clone MAC387)
| | | |
Anti-bovine TNFα
|
TNFα
|
pAb
|
1: 25
|
TC-microwaveb
|
Serotec
|
Anti-human IL-1α
|
IL-1α
|
pAb
|
1:100
|
Tween-20c
|
Endogen
|
Anti-murine iNOS/NOS Type II
|
iNOS/NOS Type II
|
pAb
|
1:100
|
TC-autoclaved
|
BD Transduction Lab.
|
Anti-murine COX-2
|
COX-2
|
pAb
|
1:75
|
TC-autoclaved
|
Cayman Chemical
|
Anti-Human Von Willebrand Factor
|
Von Willebrand Factor
|
pAb
|
1:800
|
Proteasea
|
DakoCytomation
|
- MΦs, macrophages; mAb, monoclonal antibody; pAb, polyclonal antibody.
- a Incubation with 0.1% protease type XIV (Sigma–Aldrich Chemie) in 0.01 M PBS, pH 7.2, for 10 min at room temperature.
- b Incubation with 0.1 M citric acid (pH 6), microwave for 5 min at sub-boiling temperature.
- c Incubation with 0.1% Tween 20 (Merck) in 0.01 M PBS, pH 7.2, for 10 min at room temperature.
- d Incubation with 0.1 M citric acid (pH 6) and autoclaved for 30 min at 121°C, 1 atm.