Skip to main content

Table 1 Detailed list of primary antibodies used in the immunohistochemical study

From: Pathogenic mechanisms implicated in the intravascular coagulation in the lungs of BVDV-infected calves challenged with BHV-1

Specificity

Antigen or cell detected

mAb/pAb

Dilution

Pre-treatment

Source

Anti-human MΦs

Monocytes and MΦs

mAb

1:100

Proteasea

Serotec

  

(clone MAC387)

   

Anti-bovine TNFα

TNFα

pAb

1: 25

TC-microwaveb

Serotec

Anti-human IL-1α

IL-1α

pAb

1:100

Tween-20c

Endogen

Anti-murine iNOS/NOS Type II

iNOS/NOS Type II

pAb

1:100

TC-autoclaved

BD Transduction Lab.

Anti-murine COX-2

COX-2

pAb

1:75

TC-autoclaved

Cayman Chemical

Anti-Human Von Willebrand Factor

Von Willebrand Factor

pAb

1:800

Proteasea

DakoCytomation

  1. MΦs, macrophages; mAb, monoclonal antibody; pAb, polyclonal antibody.
  2. a Incubation with 0.1% protease type XIV (Sigma–Aldrich Chemie) in 0.01 M PBS, pH 7.2, for 10 min at room temperature.
  3. b Incubation with 0.1 M citric acid (pH 6), microwave for 5 min at sub-boiling temperature.
  4. c Incubation with 0.1% Tween 20 (Merck) in 0.01 M PBS, pH 7.2, for 10 min at room temperature.
  5. d Incubation with 0.1 M citric acid (pH 6) and autoclaved for 30 min at 121°C, 1 atm.