Pathogenic mechanisms implicated in the intravascular coagulation in the lungs of BVDV-infected calves challenged with BHV-1

Table 1 Detailed list of primary antibodies used in the immunohistochemical study

*Specificity*	*Antigen or cell detected*	*mAb/pAb*	*Dilution*	*Pre-treatment*	*Source*
Anti-human MΦs	Monocytes and MΦs	mAb	1:100	Protease^a	Serotec
		(clone MAC387)
Anti-bovine TNFα	TNFα	pAb	1: 25	TC-microwave^b	Serotec
Anti-human IL-1α	IL-1α	pAb	1:100	Tween-20^c	Endogen
Anti-murine iNOS/NOS Type II	iNOS/NOS Type II	pAb	1:100	TC-autoclave^d	BD Transduction Lab.
Anti-murine COX-2	COX-2	pAb	1:75	TC-autoclave^d	Cayman Chemical
Anti-Human Von Willebrand Factor	Von Willebrand Factor	pAb	1:800	Protease^a	DakoCytomation

MΦs, macrophages; mAb, monoclonal antibody; pAb, polyclonal antibody.
^a Incubation with 0.1% protease type XIV (Sigma–Aldrich Chemie) in 0.01 M PBS, pH 7.2, for 10 min at room temperature.
^b Incubation with 0.1 M citric acid (pH 6), microwave for 5 min at sub-boiling temperature.
^c Incubation with 0.1% Tween 20 (Merck) in 0.01 M PBS, pH 7.2, for 10 min at room temperature.
^d Incubation with 0.1 M citric acid (pH 6) and autoclaved for 30 min at 121°C, 1 atm.

ISSN: 1297-9716