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Table 1 Primer pairs used for quantification of gene expression by qPCR

From: CD27 expression discriminates porcine T helper cells with functionally distinct properties

Target

Accession number

Primer sequence Forward (F), Reverse (R)

Position on + strand

Product length (bp)

Source

CD62L

NM_001112678.1

F: AGCAAAGACTCCGGGAAGTG

404

247

designed by primer3[17]

R: AGAACTTGCCCAAAGGGTGA

651

CX3CR1

XM_003358374.1

F: CGCAGGACAGGGTGGCGGAT

−67

217

designed by primer BLAST[18]

  

R: ATTGCCCACGAGGCCAAAGGC

150

  
  1. Sequences of primers (5’-3’), primer positions on (+) strand relative to the ATG start codon and product length in base pairs (bp) are indicated.
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Veterinary Research

ISSN: 1297-9716