Skip to main content

Table 5 In vitro culture of monocytes induces cell death, which is not increased by EHV-1 infection

From: Equine herpesvirus type 1 infection induces procoagulant activity in equine monocytes

Treatment Monocyte gate Dead cell gate
  % total % dead % total % dead
Vehicle 54 ± 21 16 ± 3 9 ± 7 65 ± 29
LPS 55 ± 25 18 ± 15 6 ± 4 80 ± 18
RacL11 MOI 1 59 ± 17 14 ± 8 9 ± 7 79 ± 24
RacL11 MOI 5 59 ± 14 16 ± 10 7 ± 5 83 ± 17
Sodium azide 33 ± 26 31 ± 26 18 ± 19 92 ± 10
  1. Live-dead assays with Annexin V and propidium iodide were performed on suspended monocyte-enriched fractions 24 h after EHV-1 infection with RacL11 (MOI of 1 and 5) or treatment with LPS (10 μg/mL) or vehicle (PBS). Sodium azide (25 mM) was used as a positive control for induction of cell death. The mean ± SD percentage of total cells and dead cells (expressing Annexin V or propidium iodide) was calculated in the monocyte and “dead cell” gate (R1 and R2 in Figure 5, respectively) (n = 4). Mean percentages for infected or treated cells were not significantly different from vehicle-treated controls (p > 0.05).