Treatment | Monocyte gate | Dead cell gate |
---|
| % total | % dead | % total | % dead |
---|
Vehicle | 54 ± 21 | 16 ± 3 | 9 ± 7 | 65 ± 29 |
LPS | 55 ± 25 | 18 ± 15 | 6 ± 4 | 80 ± 18 |
RacL11 MOI 1 | 59 ± 17 | 14 ± 8 | 9 ± 7 | 79 ± 24 |
RacL11 MOI 5 | 59 ± 14 | 16 ± 10 | 7 ± 5 | 83 ± 17 |
Sodium azide | 33 ± 26 | 31 ± 26 | 18 ± 19 | 92 ± 10 |
- Live-dead assays with Annexin V and propidium iodide were performed on suspended monocyte-enriched fractions 24 h after EHV-1 infection with RacL11 (MOI of 1 and 5) or treatment with LPS (10 μg/mL) or vehicle (PBS). Sodium azide (25 mM) was used as a positive control for induction of cell death. The mean ± SD percentage of total cells and dead cells (expressing Annexin V or propidium iodide) was calculated in the monocyte and “dead cell” gate (R1 and R2 in Figure 5, respectively) (n = 4). Mean percentages for infected or treated cells were not significantly different from vehicle-treated controls (p > 0.05).