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Table 5 In vitro culture of monocytes induces cell death, which is not increased by EHV-1 infection

From: Equine herpesvirus type 1 infection induces procoagulant activity in equine monocytes

Treatment

Monocyte gate

Dead cell gate

 

% total

% dead

% total

% dead

Vehicle

54 ± 21

16 ± 3

9 ± 7

65 ± 29

LPS

55 ± 25

18 ± 15

6 ± 4

80 ± 18

RacL11 MOI 1

59 ± 17

14 ± 8

9 ± 7

79 ± 24

RacL11 MOI 5

59 ± 14

16 ± 10

7 ± 5

83 ± 17

Sodium azide

33 ± 26

31 ± 26

18 ± 19

92 ± 10

  1. Live-dead assays with Annexin V and propidium iodide were performed on suspended monocyte-enriched fractions 24 h after EHV-1 infection with RacL11 (MOI of 1 and 5) or treatment with LPS (10 μg/mL) or vehicle (PBS). Sodium azide (25 mM) was used as a positive control for induction of cell death. The mean ± SD percentage of total cells and dead cells (expressing Annexin V or propidium iodide) was calculated in the monocyte and “dead cell” gate (R1 and R2 in Figure 5, respectively) (n = 4). Mean percentages for infected or treated cells were not significantly different from vehicle-treated controls (p > 0.05).