Table 1 Primers used for measurement of equine TF mRNA by quantitative real-time PCR
From: Equine herpesvirus type 1 infection induces procoagulant activity in equine monocytes
Gene | Length (bp) | Amplicon size | Sequence (5’ – 3’) |
|---|---|---|---|
Equus caballus TF forward | 23 | 243 | 5' - GTGGCTAGAGCCGCAGGGACTAG |
Equus caballus TF reverse | 21 | 243 | 5' - AGGAAGAGACCCGCGCCATGT |
Equus caballus β2-microglobulin forward | 22 | 209 | 5’ - TGTCTCTGGGTTCCATCCGCCT |
Equus caballus β2-microglobulin reverse | 25 | 209 | 5’ - CGGACCCACTTAACTATCAGGGGGT |