Western-Blot and dot blot Quantification of protein expression results in scrapie CNS tissues. Specificity of anti-CAPN6, anti-COL1A2, anti-COL3A1, anti-GALA1, anti-MTNR1B and anti-MT2A antibodies in the ovine medulla oblongata, as detected by Western blotting (A). Images of significant results of dot blot quantification (B). Protein expression profiles of the six scrapie-related proteins (i.e., CAPN6, COL1A2, COL3A1, GALA1, MTNR1B and MT2A) in four CNS regions in control (white bars), preclinical scrapie (grey bars) and clinical scrapie (black bars) sheep in the cervical spinal cord, medulla oblongata, cerebellar cortex and diencephalon using the dot blot assay (C). Density of immunoreactive dots was normalized for Ponceau Red and reported as arbitrary units (a.u.). Data are expressed as means ± standard error. Differences between groups were analyzed using a Student’s t-test (*P < 0.05 and **P < 0.01).