Expression of PToV-HE52.7 and PToV-HE52.11 proteins. (A) Western blot detection of HE proteins in extracts from BSC40 cells infected (MOI 5) with rVV-HE52.7, rVV-HE52.11 or rVV-HA-. Infected cells were fractionated by 10% SDS-PAGE, transferred to a nitrocellulose membrane and reacted with the αHE antibody. α-Tubulin signalling was taken along as a loading standard. Molecular size markers are given in kDa. (B) Immunofluorescence microscopy of BSC40 cells infected with rVV (MOI 5). Cells were fixed at 7 hpi, permeabilized and incubated with αHE antibody followed by a secondary antibody coupled to Alexa Fluor 594 (red). Cell nuclei were stained using DAPI (blue). Scale bar: 10 μm.